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MB Sample ID: SA150495
| Local Sample ID: | Controlinvivo_10 |
| Subject ID: | SU001715 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 4 weeks mice |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
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Collection:
| Collection ID: | CO001708 |
| Collection Summary: | A549 lung cancer xenograft tumors were obtained after sacrificing the mice. The tumors were then prepared according to the protocol described and metabolomics analysis was performed. |
| Sample Type: | Tumor cells |
| Collection Method: | Tumors were homogenized in a Beadbud homogenizer (Benchmark scientific) in a mixture of LC-MS grade ice-cold methanol and water (1:1; v/v). The supernatant was collected and sonicated in a water bath incubator for 15 minutes, followed by centrifugation at 13,000 rpm for 10 minutes and collection of the supernatant. |
| Collection Location: | Homogenization vials |
| Collection Frequency: | Once |
| Collection Duration: | 4-5 minutes |
| Volumeoramount Collected: | 1ml |
| Storage Conditions: | -80℃ |
| Collection Vials: | Polypropylene 1.5 ml tubes |
| Storage Vials: | Polypropylene 1.5 ml |
