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MB Sample ID: SA162056
| Local Sample ID: | MH3_2 |
| Subject ID: | SU001798 |
| Subject Type: | Invertebrate |
| Subject Species: | Cherax tenuimanus |
| Taxonomy ID: | 99755 |
| Weight Or Weight Range: | 116g-198g |
| Gender: | Male and female |
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Collection:
| Collection ID: | CO001791 |
| Collection Summary: | All haemolymph collections for all animals occurred at the same time of day and the animals were handled in the same order on each of the four collection dates. Haemolymph (200mL) was extracted from the ventral sinus of each crayfish using a 21G needle and 1mL syringe inserted into the soft tissue at the base of the 5th pereopod. Haemolymph was added to 2mL Eppendorf tubes preloaded with 600µL LC-MS grade acetonitrile (Optima, Thermo Fisher Scientific, AUS) containing deuterated internal standards (d8- valine, d9 –trimethylamine-N-oxide (TMAO) , d3-leucine, d6-trans-cinnamic acid, d5-tryptophan, 1g/mL) from Cambridge Isotope Laboratories (Cambridge, MA, USA) and stored on ice. Samples were mixed at 1400rpm (Thermal Mixer, Thermo Fisher Scientific, AUS) for 60 seconds at 4°C, then centrifuged (Heraeus Megafuge 8R, Thermo Fisher Scientific, AUS) for 20 minutes (1800 g) at 4°C. After centrifuging, 100µL of supernatant was aliquoted into five separate vials. The samples were then dried using a SpeedVac centrifugal vacuum concentrator (Thermo Fisher Scientific, AUS). The dried samples were stored at -80°C for subsequent metabolomics analysis. |
| Collection Protocol Filename: | EmilyLette_20210225_014138_PR_CO_Methods__Lette_marron.pdf |
| Sample Type: | Hemolymph |
| Collection Method: | aspiration |
| Collection Location: | ventral sinus accessed from soft tissue at base of 5th pereopod |
| Storage Conditions: | -80℃ |
| Collection Vials: | 2mL Eppendorf tubes |
| Storage Vials: | 2mL Eppendorf tubes |
