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MB Sample ID: SA167773
| Local Sample ID: | Dm_polpos_LCO_1_48 |
| Subject ID: | SU001884 |
| Subject Type: | Invertebrate |
| Subject Species: | Daphnia magna |
| Taxonomy ID: | 35525 |
| Age Or Age Range: | neonates < 9 h old |
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Collection:
| Collection ID: | CO001877 |
| Collection Summary: | Daphnid cultures D. magna were cultured at a density of 20 animals per liter MHRW, 16 h:8 h light:dark photoperiod, and a temperature of 20 °C. For metabolomics, cultures were fed daily on suspensions of unicellular green alga, Chlorella vulgaris (7.84 × 107 cells/mL) at 2 mL/L. Algae was supplemented daily by 50 µL/L of dried bakers yeast (1mg/mL stock, Sigma–Aldrich). Cultures were maintained using third or fourth brood neonates less than 24 h old. Ten replicates were prepared for each treatment: MHRW control, 1 mg/L LCO, and ion control (66 μg/L Li as LiCl and 15 μg/L Co as CoCl2; equivalent to ions released by 1 mg/L LCO over 48 h).1,2 Twenty neonates per replicate (< 9 h old) were transferred to 100 ml beakers 48 hours prior to exposure and fed proportionate amounts of food for 48 hours. At the end of the 48-hour feeding period, daphnids were transferred to 200 ml control or exposure beakers. Exposures were carried out for 48 h without food per standard OECD guidelines for D. magna. At the end of the exposure daphnids were collected and transferred (20 pooled animals per replicate) into labeled Precellys tubes using a fine sable brush and flash frozen in liquid nitrogen.At the end of the exposure daphnids were collected and transferred (20 pooled animals per replicate) into labeled Precellys tubes using a fine sable brush and flash frozen in liquid nitrogen. Samples were stored at -80 °C and shipped to the University of Birmingham, UK on dry ice. |
| Sample Type: | Whole animals |
| Storage Conditions: | -80℃ |
| Collection Vials: | Precellys tubes |
