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MB Sample ID: SA028689

Local Sample ID:264516
Subject ID:SU000574
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male
Human Race:White
Human Inclusion Criteria:blunt trauma without isolated head injury, defined as either abbreviated injury score (AIS) head <4 OR Glasgow Coma Scale (GCS) motor >3 within 24 hours of injury, emergency department arrival within 6 hours from time of injury, blood transfusion within 12 hours of injury, base deficit of at least 6 or systolic blood pressure <90 mmHg within 60 minutes of emergency department arrival, and fully or partially intact cervical spinal cord
Human Exclusion Criteria:age <16 years, anticipated survival of <24 hours from injury, anticipated survival <28 days due to pre-existing medical condition, inability to obtain first blood draw within first 12 hours after injury, traumatic brain injury (i.e., GCS ≤8 after ICU admission AND brain computerized tomography scan abnormality within 12 hours after injury), immunosuppression, significant pre-existing organ dysfunction, and receiving home oxygen therapy
Species Group:Human

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Combined analysis:

Analysis ID AN000843 AN000844
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific Q-Exactive Thermo Scientific Q-Exactive
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

MS:

MS ID:MS000744
Analysis ID:AN000843
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000745
Analysis ID:AN000844
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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