Return to study ST001130 main page

MB Sample ID: SA078086

Local Sample ID:Pos_hyper2incluMS2
Subject ID:SU001191
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN001855
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column SeQuant ZIC-HILIC (100 x 2.1mm,3.5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units Area under the curve Arbitrary units

MS:

MS ID:MS001715
Analysis ID:AN001855
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:LC-MS data from ddMS2 measurement were converted to mzML format using compound discoverer (version 2.1) (Thermo Scientific, Bellefonte, PA, USA) Data were analyzed using XCMS online tool(Forsberg et al, 2018). The metabolites were mapped onto the mouse database available on XCMS(Forsberg et al, 2018). Data was also analyzed with MSdial tool to confirm some of the metabolite annotations, using library provided with the MSdial software(Lai et al, 2017; Tsugawa et al, 2015). Forsberg EM, Huan T, Rinehart D, Benton HP, Warth B, Hilmers B, Siuzdak G (2018) Data processing, multi-omic pathway mapping, and metabolite activity analysis using XCMS Online. Nat Protoc 13: 633-651 Lai Z, Tsugawa H, Wohlgemuth G, Mehta S, Mueller M, Zheng Y, Ogiwara A, Meissen J, Showalter M, Takeuchi K, Kind T, Beal P, Arita M, Fiehn O (2017) Identifying metabolites by integrating metabolome databases with mass spectrometry cheminformatics. Nature methods 15: 53 Tsugawa H, Cajka T, Kind T, Ma Y, Higgins B, Ikeda K, Kanazawa M, VanderGheynst J, Fiehn O, Arita M (2015) MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis. Nature methods 12: 523-526
Ion Mode:POSITIVE
  logo