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MB Sample ID: SA078090
Local Sample ID: | Pos_FG2incluMS2 |
Subject ID: | SU001191 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001855 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | Area under the curve Arbitrary units |
MS:
MS ID: | MS001715 |
Analysis ID: | AN001855 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | LC-MS data from ddMS2 measurement were converted to mzML format using compound discoverer (version 2.1) (Thermo Scientific, Bellefonte, PA, USA) Data were analyzed using XCMS online tool(Forsberg et al, 2018). The metabolites were mapped onto the mouse database available on XCMS(Forsberg et al, 2018). Data was also analyzed with MSdial tool to confirm some of the metabolite annotations, using library provided with the MSdial software(Lai et al, 2017; Tsugawa et al, 2015). Forsberg EM, Huan T, Rinehart D, Benton HP, Warth B, Hilmers B, Siuzdak G (2018) Data processing, multi-omic pathway mapping, and metabolite activity analysis using XCMS Online. Nat Protoc 13: 633-651 Lai Z, Tsugawa H, Wohlgemuth G, Mehta S, Mueller M, Zheng Y, Ogiwara A, Meissen J, Showalter M, Takeuchi K, Kind T, Beal P, Arita M, Fiehn O (2017) Identifying metabolites by integrating metabolome databases with mass spectrometry cheminformatics. Nature methods 15: 53 Tsugawa H, Cajka T, Kind T, Ma Y, Higgins B, Ikeda K, Kanazawa M, VanderGheynst J, Fiehn O, Arita M (2015) MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis. Nature methods 12: 523-526 |
Ion Mode: | POSITIVE |