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MB Sample ID: SA169110

Local Sample ID:10
Subject ID:SU001897
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Not applicable

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Combined analysis:

Analysis ID AN002954 AN002955 AN002956
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase HILIC
Chromatography system Agilent 1260 Agilent 1260 Agilent 1260
Column Agilent Eclipse XDB-C18 (100 x 3.0mm) Agilent Eclipse XDB-C18 (100 x 3.0mm) Phenomenex Luna NH2 (150 x 2.1mm,3um)
MS Type ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ Agilent 6490 QQQ
Ion Mode NEGATIVE POSITIVE POSITIVE
Units area under the curve area under the curve area under the curve

MS:

MS ID:MS002744
Analysis ID:AN002954
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent 6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through three separate chromatographic conditions (reverse-phase negative mode, reverse-phase positive mode and normal-phase positive mode) for the effective quantification of over 337 distinct lipids from over 28 lipid subclasses, as previously described (41). Individual lipid species were measured by multiple reaction monitoring transitions.
Ion Mode:NEGATIVE
  
MS ID:MS002745
Analysis ID:AN002955
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent 6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through three separate chromatographic conditions (reverse-phase negative mode, reverse-phase positive mode and normal-phase positive mode) for the effective quantification of over 337 distinct lipids from over 28 lipid subclasses, as previously described (41). Individual lipid species were measured by multiple reaction monitoring transitions.
Ion Mode:POSITIVE
  
MS ID:MS002746
Analysis ID:AN002956
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent 6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through three separate chromatographic conditions (reverse-phase negative mode, reverse-phase positive mode and normal-phase positive mode) for the effective quantification of over 337 distinct lipids from over 28 lipid subclasses, as previously described (41). Individual lipid species were measured by multiple reaction monitoring transitions.
Ion Mode:POSITIVE
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