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MB Sample ID: SA218462
| Local Sample ID: | WT3 |
| Subject ID: | SU002366 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003724 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 7250 GC/Q-TOF |
| Ion Mode | POSITIVE |
| Units | relative abundance |
MS:
| MS ID: | MS003472 |
| Analysis ID: | AN003724 |
| Instrument Name: | Agilent 7250 GC/Q-TOF |
| Instrument Type: | QTOF |
| MS Type: | EI |
| MS Comments: | The EI source was operated at 70 eV whereas the mass spectrometer operated in the scan mode over a mass range of m/z 50–600. Metabolite deconvolution and identification were carried out using Agilent MassHunter Unknowns Analysis version B.07.00, then, data was aligned in Agilent Mass Profiler Professional version B.12.1 and exported to Agilent MassHunter Quantitative Analysis version B.07.00. Metabolites were identified by comparing their retention time, retention index and mass fragmentation patterns with those available in an in-house library including both the NIST mass spectral database (version 2017) and Fiehn RTL library (version 2008). The different derivatives that were generated from the silylated compounds were unified by summing the abundance of all derivatives from the same metabolite. Finally, the median relative area of the two analytical replicates of the same sample was computed and used for subsequent statistical analysis. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Protocol_AM.pdf |
