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MB Sample ID: SA094082
| Local Sample ID: | 233_17 |
| Subject ID: | SU001368 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 32-67 |
| Gender: | Male and female |
| Human Race: | Caucasian (C) and African American (AA) |
| Human Ethnicity: | non-hispanic |
| Human Inclusion Criteria: | Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc) |
| Human Exclusion Criteria: | Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent. |
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NMR:
| NMR ID: | NM000157 |
| Analysis ID: | AN002155 |
| Instrument Name: | Bruker 18.8 Tesla (800 MHz) NMR spectrometer ascend |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| NMR Comments: | Arrayed for water saturation frequency and 90deg. pulse width for each sample |
| Standard Concentration: | 10% (~0.5mM) |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | Triple resonance inverse detection TCI cryoprobe |
| NMR Solvent: | D20 |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming) |
| Pulse Sequence: | noesygppr1d |
| Water Suppression: | saturation at 80 Hz induced field strength |
| Pulse Width: | 5.5ms |
| Offset Frequency: | around -178Hz |
| Presaturation Power Level: | 80db |
| Chemical Shift Ref Cpd: | DSS-d6 |
| Temperature: | 25 |
| Number Of Scans: | 128 |
