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MB Sample ID: SA001593
| Local Sample ID: | 131221ajcsa25_1 |
| Subject ID: | SU000042 |
| Subject Type: | Insect |
| Subject Species: | Centroptilum triangulifer;Atherix;Megaloptera |
| Taxonomy ID: | 2078957;124299;50553 |
| Species Group: | Insect |
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Sample Preparation:
| Sampleprep ID: | SP000038 |
| Sampleprep Summary: | Insect samples homogenized in 50% acetonitrile at a concentration of 50 mg/mL. Sample dried and aliquot taken for NMR. Sample returned and extract processed for GC/MS analysis. Samples were derivatized using 2 step derivatization method (methoximation with methoxy amine followed by TMS derivatization with MSTFA). |
| Sampleprep Protocol Filename: | RTI_DB_INSECT_Metabolomics_Procedure_March12_2014.docx |
| Processing Method: | Homogenization and Solvent Removal with SpeedVac |
| Processing Storage Conditions: | 4C |
| Extraction Method: | 50% Acetonitrile |
| Extract Concentration Dilution: | dried on SpeedVac |
| Extract Storage: | -80C |
| Sample Resuspension: | resuspended in D2O + Chenomix ISTD solution |
| Sample Derivatization: | Methoximation with methoxy amine at 40 mg/ml in pyridine followed by TMS derivatization with MSTFA |
| Sample Spiking: | FAMES (C8-C30) added to MSTFA as retention time markers |
