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MB Sample ID: SA002249
| Local Sample ID: | C-194 |
| Subject ID: | SU000069 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Infant |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000065 |
| Sampleprep Summary: | Fifty (50) µl of 0.9% NaCl solution containing 4mM sodium formate (Chemical Shift Indicator) and 0.2% NaN3 (to inhibit bacterial growth) in D2O was pipetted into each of the 50 serum samples (150 µl aliquots). Each 200 µl sample was vortexed for 30 seconds and transferred into 3mm NMR tubes (Bruker Bio-Spin, Germany). Pooled serum samples for each phenotype were made by combining 12 µl aliquots from each of the study samples in the phenotypic group (low and high) into two separate eppendorf tubes. A combined total pooled sample was also prepared by combining 6 µl aliquots from all samples into an eppendorf tube. The pooled samples were prepared as described above and were used as quality check (QC) samples. |
| Sampleprep Protocol Filename: | Fetal_Metabolomics_of_iAs_exposure_Metabolomics_Procedure.docx |
| Processing Method: | Dilution with D2O containing 0.9% NaCl and 4 mM Formate |
| Processing Storage Conditions: | On ice |
