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MB Sample ID: SA002793
| Local Sample ID: | 1402110jcsa41_1 |
| Subject ID: | SU000073 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | female |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000069 |
| Sampleprep Summary: | Breast tissue samples were homogenized in 3:3:2 (acetonitrile:isopropanol:water) at a concentration of 100 mg/mL. Samples dried and extracted into 50% acetonitrile. Sample dried and aliquot taken for NMR. Sample returned and extract processed for GC/MS analysis. Samples were derivatized using 2 step derivatization method (methoximation with methoxy amine followed by TMS derivatization with MSTFA). |
| Sampleprep Protocol Filename: | RTI_MT_Breast_Tissue_Procedures_GC-TOF-MS.docx |
| Processing Method: | Homogenization and Solvent Removal with SpeedVac |
| Processing Storage Conditions: | 4°C |
| Extraction Method: | 3:3:2 (acetonitrile:isopropanol:water) and then 50% Acetonitrile |
| Extract Concentration Dilution: | dried on SpeedVac |
| Extract Storage: | -80°C |
| Sample Resuspension: | resuspended in D2O + Chenomix ISTD solution |
| Sample Derivatization: | Methoximation with methoxy amine at 40 mg/ml in pyridine followed by TMS derivatization with MSTFA |
| Sample Spiking: | FAMES (C8-C30) added to MSTFA as retention time markers |
