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MB Sample ID: SA002894
| Local Sample ID: | F_Dirty_1575 |
| Subject ID: | SU000075 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C3H/HeN |
| Gender: | Female |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000071 |
| Sampleprep Summary: | Frozen fecal samples were transferred into numbered homogenizer bead tubes (ceramic) and diluted with D2O (Sigma-Aldrich, St. Louis, MO). Specimens with a mass of less than 100 mg were diluted with 0.5 mL; specimens with a mass of greater than 100 mg were diluted with 1.0 mL. The samples were then homogenized using the MagNA Lyser bead homogenizer for two 30 second pulses at 2000 rpm and then vortexed for an additional 30 seconds. The samples were pelleted by centrifugation at 12000 rcf for 5 minutes. 50 mg of extract were utilized for each specimen, and combined the fecal extract and D2O were a total volume of 630 µL. 70 µL of Chenomx ISTD solution into all tubes. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, chemical shift indicator), 100 mM imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 µL was transferred into each NMR tube for analysis. |
| Sampleprep Protocol Filename: | RTI_Food_Allergy_Envirnomental_Metabolomics_Procedure.docx |
