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MB Sample ID: SA006742
| Local Sample ID: | S48 |
| Subject ID: | SU000140 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 20 weeks |
| Weight Or Weight Range: | 26.8 +/- 2.2 g |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Labs (Bar Harbor, ME) |
| Animal Housing: | 8 / cage |
| Animal Light Cycle: | 12 h / 12 h light/dark |
| Animal Feed: | Standard chow, ad lib. |
| Animal Water: | Ad lib. Access |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000137 |
| Sampleprep Summary: | Frozen tissue samples were pulverized in a liquid nitrogen-chilled mortar and pestle, then sonicated in cold extraction solvent (7:2:1 methanol:chloroform:water) at an approximate ratio of 30 mg tissue (wet mass) /1 mL of solvent. After allowing samples to rest on ice 5 minutes, they were centrifuged to pellet precipitated protein and cell debris; the supernatant was placed in autosampler vials for direct LC-MS analysis. For serum, extraction solvent (1:1:1 methanol:acetonitrile:acetone) was added to serum at a 4:1 solvent:serum ratio, vortexed to mix, then centrifuged and supernatant recovered for LC-MS analysis. |
| Sampleprep Protocol Filename: | PROTOCOLS_mouse_anesthesia.pdf |
| Processing Method: | Cryo-pulverization + sonication in extraction solvent |
