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MB Sample ID: SA011068

Local Sample ID:man10
Subject ID:SU000256
Subject Type:Human/animal
Subject Species:Homo sapiens;Rattus norvegicus
Taxonomy ID:9606;10116
Genotype Strain:Sprague-Dawley (Rat)
Age Or Age Range:23-29 (Human)
Weight Or Weight Range:220-250g (Rat)
Gender:Male/Female (Human and Rat)
Human Race:Yellow Race (Human)
Human Inclusion Criteria:Post-graduate students (Human)
Animal Housing:pathogen-free animal facility (Rat)
Animal Light Cycle:12h light/dark cycle (Rat)
Animal Feed:free access to standard food (Rat)
Animal Water:free access to water (Rat)
Species Group:Mammal

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Sample Preparation:

Sampleprep ID:SP000260
Sampleprep Summary:Amygdalin was dissolved in acetonitrile-water(1:1) to form 0.1mg/ml solution. Urine samples were unfrozen at room temperature. 600?l of urine sample was mixed with 20?l Amygdalin solution. After centrifugation at about 14000rpm and -4?, the supernatant was injected into UPLC-TOF MS system.
Sampleprep Protocol Filename:2_PRE_PROT.pdf
Processing Method:Centrifugation
Processing Storage Conditions:Centrifugation at -4?
Extraction Method:Centrifugation
Extract Concentration Dilution:600?l of urine/620?l
Extract Storage:4? after centrifugation and during analysis
Sample Spiking:20?l Amygdalin solution/620?l
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