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MB Sample ID: SA011082
| Local Sample ID: | MaleRat3 |
| Subject ID: | SU000257 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley rat |
| Weight Or Weight Range: | 220-250g |
| Gender: | Male/Female |
| Animal Housing: | pathogen-free animal facility |
| Animal Light Cycle: | 12h light/dark cycle |
| Animal Feed: | free access to standard food |
| Animal Water: | free access to water |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000261 |
| Sampleprep Summary: | Amygdalin was dissolved in acetonitrile-water(1:1) to form 0.1mg/ml solution. Serum samples were unfrozen at room temperature. 500?l of serum sample was mixed with 20?l Amygdalin solution and 100?l 20% Trichloroacetic acid(TCA) solution. After centrifugation at about 14000rpm and -4?, the supernatant was injected into UPLC-TOF MS system. |
| Sampleprep Protocol Filename: | 2_PRE_PROT.pdf |
| Processing Method: | Precipitation |
| Processing Storage Conditions: | Precipitation at room tempreture; centrifugation at -4? |
| Extraction Method: | Precipitation by TCA |
| Extract Concentration Dilution: | 500?l of serum/620?l |
| Extract Storage: | 4? after centrifugation and during analysis |
| Sample Spiking: | 20?l Amygdalin solution/620?l |
