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MB Sample ID: SA011336
| Local Sample ID: | folch_high |
| Subject ID: | SU000266 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC - Supplier |
| Subject Comments: | P?+10 |
| Cell Primary Immortalized: | immortalized |
| Cell Passage Number: | P?+10 |
| Cell Counts: | P?+10 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000271 |
| Sampleprep Summary: | Cells were extracted using either the Folch or Matyash method. The total volume of MeOH in each extraction method was varied (0.5 mL or 2.0 mL) while the ratio of MeOH to the other solvents was maintained. |
| Sampleprep Protocol Filename: | SOP_LipidExtractionProtocol.pdf |
| Processing Method: | cell lysis |
| Processing Storage Conditions: | on ice |
| Extraction Method: | Folch or Matyash lipid extraction method |
| Extract Concentration Dilution: | 1e6 cells were extracted with the solvent systems explained in the Sample Prep Summary |
| Sample Resuspension: | 100 μL of IPA |
| Sample Spiking: | internal standards: lysophosphatidylcholine (LPC 17:0 and LPC 19:0), phosphatidylcholine (PC 17:0/17:0 and PC 19:0/19:0), phosphatidylethanolamine (PE 15:0/15:0 and PE 17:0/17:0), phosphatidylserine (PS 14:0/14:0 and PS 17:0/17:0), and phosphatidylglycerol (PG 14:0/14:0 and PG 17:0/17:0), triacylglyceride (TG 15:0/15:0/15:0 and TG 17:0/17:0/17:0) |
| Cell Type: | Jurkat, Clone E6-1 |
