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MB Sample ID: SA011542
| Local Sample ID: | WEDG-1 |
| Subject ID: | SU000273 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Age Or Age Range: | 2 weeks |
| Animal Housing: | Litters of 10 (N group) or 17 (R group) |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000279 |
| Sampleprep Summary: | Tissues were pulverized on dry ice, enriched with deuterated surrogates, antioxidants (BHT and EDTA), and the total lipid extract was isolated using 10:8:11 cyclohexane/2-propanol/ammonium acetate (v/v/v). Subsequently, esterified oxylipins were liberated via sodium methoxide. Next, the remaining extract was eluted through a Waters Oasis-HLB cartridge. Plasma was added directly to a Waters Ostro plate, mixed with the surrogates, antioxidants, and acetonitrile and the extract eluted through. Both tissue and plasma extracts were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal standards 1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm. |
| Sampleprep Protocol Filename: | NewmanLab-F&BOxys-Protocol-PT.pdf |
| Extraction Method: | Liquid:liquid with HLB solid phase extraction (Tissue); Ostro plate (Plasma) |
| Extract Cleanup: | HLB solid phase extraction (Tissue); Ostro plate (Plasma) |
| Extract Storage: | - 20 °C |
| Sample Resuspension: | 100 µL |
| Sample Spiking: | See sample prep protocol file |
| Organ: | Lung and plasma |
