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MB Sample ID: SA011743
| Local Sample ID: | SW epi 3 |
| Subject ID: | SU000277 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 30 weeks |
| Gender: | Female |
| Animal Housing: | Singly housed |
| Animal Feed: | High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet (D12450, Research Diets, New Brunswick, NJ) |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000283 |
| Sampleprep Summary: | Tissues were pulverized on dry ice, enriched with deuterated surrogates, antioxidants (BHT and EDTA), and extracted with MeOH:Water:IPA, transferred to an Ostro plate and the extract was eluted by vacuum. Plasma was added directly to the plate, mixed with the surrogates, antioxidants, and IPA and the extract eluted through. The extracts were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal standards 1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm. |
| Sampleprep Protocol Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Extraction Method: | Ostro plate |
| Extract Concentration Dilution: | 80 µL |
| Extract Cleanup: | Ostro plate |
| Extract Storage: | - 20 °C |
| Sample Resuspension: | 100 µL |
| Sample Spiking: | See sample prep protocol file |
| Organ: | Liver, adipose, hypothalamus, muscle, and plasma |
