Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA014464

Local Sample ID:	ND6
Subject ID:	SU000336
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP000344
Sampleprep Summary:	For blood collection, the mice were sacrificed by cardiac puncture. Plasma (100 µL) was subjected to extraction with cold methanol (500 µL) on a MM400 mixer mill (Retsch®, Haan, Germany) with the frequency 30 s?1 for 5 min. The suspension was centrifuged at 12,578 g for 10 min at 4°C. The supernatant was filtered through a 0.2-µm PTEE filter and evaporated in a speed vacuum concentrator. The final concentration of the plasma was 5 mg/mL in methanol for the UPLC-Q-TOF-MS analysis.

Sampleprep ID:

SP000344

Sampleprep Summary:

For blood collection, the mice were sacrificed by cardiac puncture. Plasma (100 µL) was subjected to extraction with cold methanol (500 µL) on a MM400 mixer mill (Retsch®, Haan, Germany) with the frequency 30 s?1 for 5 min. The suspension was centrifuged at 12,578 g for 10 min at 4°C. The supernatant was filtered through a 0.2-µm PTEE filter and evaporated in a speed vacuum concentrator. The final concentration of the plasma was 5 mg/mL in methanol for the UPLC-Q-TOF-MS analysis.