Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA025770

Local Sample ID:	W-12
Subject ID:	SU000518
Subject Type:	LC-MS & GC-MS
Subject Species:	Nicotiana tabacum
Taxonomy ID:	4097
Species Group:	Plant

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Sample Preparation:

Sampleprep ID:	SP000525
Sampleprep Summary:	Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS.

Sampleprep ID:

SP000525

Sampleprep Summary:

Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS.