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MB Sample ID: SA059437
| Local Sample ID: | RB14 H03 |
| Subject ID: | SU001016 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
| Human Race: | Hispanic and Caucasian |
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Sample Preparation:
| Sampleprep ID: | SP001023 |
| Sampleprep Summary: | After sample randomization, 50 µl plasma aliquots were enriched with deuterated surrogates in 10 µL methanol (Table S1 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 200 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol:acetonitrile was added. Protein precipitate and debris were removed by centrifugation for 10 min at 4,500 RCF. The supernatant was filtered by centrifugation through 0.2 µm PVDF membranes (Agilent Technologies, Santa Clara, CA, USA) at 500 RCF for 5 min. |
| Sampleprep Protocol Filename: | V_Medici_Plasma_Oxy-EC_analysis_protocol.pdf |
