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MB Sample ID: SA060040
| Local Sample ID: | 17 |
| Subject ID: | SU001021 |
| Subject Type: | Fish |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
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Sample Preparation:
| Sampleprep ID: | SP001028 |
| Sampleprep Summary: | Fifty μl of protein precipitant (containing NVL) was added to 50 μl of serum solution of each sample, mixed thoroughly, and centrifuged at 13200 rpm for 4 min. The supernatant (10 μl) was mixed with 50 μl methanol borate buffer (0.1 M, pH=8.8) thoroughly. For amino acids, the above solution was mixed with 20 μl of diethyl ethoxymethylene-malonate at 55°C for 15 min. |
