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MB Sample ID: SA063436
| Local Sample ID: | C3 |
| Subject ID: | SU001047 |
| Subject Type: | Other |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP001054 |
| Sampleprep Summary: | 600 µL of bacteria suspension after 4h incubation was centrifuged (700 g, 4 °C for 1 min) and supernatants were transferred to a new tube, centrifuged (6000 g, 4 °C for 3 min) and washed 3 times with PBS. After the final centrifugation, 1 mL cold 50% aqueous methanol containing 1 µM chlorpropamide and 1.0 mm diameter zirconia/silica beads (BioSpec, Bartlesville, OK) were added to the microbial pellet, followed with homogenization (6500 rpm, 1 cycle, 60 s). The sample was freeze-thawed three times with liquid nitrogen to break apart tough microbial cell wall. Then the sample was centrifuged (max speed, 4 °C, and 10 min), supernatants were transferred to a new EP tube, dried down and resuspended in 200 µL 3% aqueous methanol. After a final spin (max speed, 4 °C, and 10 min), 150 µL supernatants were transferred to autosampler for LC-MS analysis. |
| Sampleprep Protocol ID: | LC sample prep.docx |
| Sampleprep Protocol Filename: | LC_sample_prep.docx |
