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MB Sample ID: SA092108
| Local Sample ID: | GLD057 |
| Subject ID: | SU001336 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP001344 |
| Sampleprep Summary: | 100 µL of plasma spiked with internal standards underwent a protein crash with 400 µL ice cold methanol. The supernatant was dried under nitrogen. Methyl tert-butyl ether (MTBE) and water were added to extract the hydrophobic and hydrophilic compounds, respectively. The upper hydrophobic layer was transferred to a new tube and the lower hydrophilic layer was re-extracted with MTBE. The upper hydrophobic layer was combined, dried under nitrogen and reconstituted in 200 µL of methanol. The hydrophilic layer was dried under nitrogen, underwent a second protein crash with water and ice-cold methanol (1:4 water-methanol). The supernatant was removed, dried by SpeedVac at 45 °C and reconstituted in 100 µL of 5% acetonitrile in water. Both fractions were stored at -80 °C until LCMS analysis. |
| Processing Storage Conditions: | On ice |
