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MB Sample ID: SA100677
| Local Sample ID: | 2019_09_13_RPIP15_Ref-Media-H1-M5_1 |
| Subject ID: | SU001451 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP001459 |
| Sampleprep Summary: | The metabolite extraction was started using LC-MS or HPLC grade methanol (Sigma-Aldrich, 1.06035). Briefly, a 950 μL of pre-chilled 50% MeOH/H2O was added to a 50 μL of the bioreactor-collected media (making a D20 dilution), and the samples were incubated on ice for 30 min to allow for full extraction. Macromolecules were then pelleted by centrifugation at max speed (~18,000-21,000 g) for 10 mins in a bench top centrifuge (preferably chilled) to extract the supernatant. Further, the extracted samples were stored in -80 prior to running HPLC-MS. |
| Processing Storage Conditions: | Room temperature |
| Extraction Method: | 50% Methanol in water |
| Extract Storage: | On ice |
