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MB Sample ID: SA118531

Local Sample ID:ArcA_2
Subject ID:SU001495
Subject Type:Bacteria
Subject Species:Escherichia coli
Taxonomy ID:562
Genotype Strain:K-12 MG1655

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Sample Preparation:

Sampleprep ID:SP001503
Sampleprep Summary:Samples were extracted from the Wildtype, fnr, arcA and ihf mutants grown in a bioreactor anaerobically in glucose minimal media at 37 degrees Celsius and 150 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects.
Sampleprep Protocol Filename:ankita_pal_MS-method_msi.pdf
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