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MB Sample ID: SA123771
| Local Sample ID: | 12_B1-2_Colon_syngenic_7days_170427_UKy_GCH_rep3-polar-ICMS_A |
| Subject ID: | SU001521 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP001529 |
| Sampleprep Summary: | Before going into the IC-FTMS the frozen sample is reconstituted in water. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Polar extraction from homogenate, lypholized, and frozen. Protein extraction and quantification. Tissue is frozen in liquid nitrogen to stop metabolic processes. |
| Sampleprep Protocol ID: | IC-FTMS_preparation frozen_tissue_grind polar_extraction protein_extraction tissue_quench |
| Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D_17Jun4_Fan_Prot_Quant.pdf 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf |
