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MB Sample ID: SA124978
Local Sample ID: | AC2 43 (3) |
Subject ID: | SU001553 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
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Sample Preparation:
Sampleprep ID: | SP001561 |
Sampleprep Summary: | Brain regions were homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a biphasic Bligh–Dyer extraction method (methanol/water/chloroform, 2:1.8:2). The polar phases of biphasic extracts were used for LC-MS/MS analysis |