Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA125837

Local Sample ID:	56
Subject ID:	SU001565
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Female

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP001573
Sampleprep Summary:	Urine aliquots were prepared and analyzed in a random order as previously described (Contrepois et al., 2015). Briefly, frozen urine samples were thawed on ice and centrifuged at 17,000g for 10 min at 4°C. Supernatants (25 µl) were then diluted 1:4 with 75% acetonitrile and 100% water for HILIC- and RPLC-MS experiments, respectively. Each sample was spiked-in with 15 analytical-grade internal standards (IS). Samples for HILIC-MS experiments were further centrifuged at 21,000g for 10 min at 4°C to precipitate proteins.

Sampleprep ID:

SP001573

Sampleprep Summary:

Urine aliquots were prepared and analyzed in a random order as previously described (Contrepois et al., 2015). Briefly, frozen urine samples were thawed on ice and centrifuged at 17,000g for 10 min at 4°C. Supernatants (25 µl) were then diluted 1:4 with 75% acetonitrile and 100% water for HILIC- and RPLC-MS experiments, respectively. Each sample was spiked-in with 15 analytical-grade internal standards (IS). Samples for HILIC-MS experiments were further centrifuged at 21,000g for 10 min at 4°C to precipitate proteins.