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MB Sample ID: SA137450
| Local Sample ID: | U_442 |
| Subject ID: | SU001695 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-75 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP001701 |
| Sampleprep Summary: | Urine samples were allowed to thaw at 4 °C overnight. All samples were vortexed via multiple tube vortex for 2 min at 3000 rpm. The urine were spin downed using centrifuge at 1000 rpm for 30 sec. A volume of 7 µL aliquot of all 298 samples were transferred into one 10.0 mL tube labeled as pooled. A volume of 50 µL of urine (including study pool) was transferred to a new, pre-labeled Lo-Bind Eppendorf tube. A volume of 400 µL Protein Precipitation Buffer with Internal Standard (500 ng/ml Tryptophan-d5 in methanol) was added to each tube. All individual samples were thoroughly mixed on multiple tube vortexer for 2 mins at 5000 rpm. The protein precipitate was palleted using a centrifuge operating at room temperature and at 16,000 rcf for 4 min. A volume of 350 µl of the supernatant was transferred into pre-labeled 2.0 ml Low-bind Eppendorf tube. Sample were dried using speed vac overnight. A volume of 100 µL of reconstitution buffer (95:5 Water-Methanol) was added to each sample. Samples were thoroughly mixed on multiple tube vortexer for 10 mins at 5000 rpm. Samples were centrifuged at 4°C and at 16,000 rcf for 10 min. The supernatant was transferred to pre-labeled autosampler vials. A volume of 5 µL was injected onto column. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | Described in summary |
