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MB Sample ID: SA137873
| Local Sample ID: | CP_26 |
| Subject ID: | SU001696 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-75 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP001702 |
| Sampleprep Summary: | Aliquots of each de-identified sample were shipped to the NIH ERCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 298 study urine samples were thawed at 4°C overnight. Aliquots of 400 µL of study samples were transferred to 2.0mL pre-labeled LoBind Eppendorf tubes. Aliquots of 400 µL of analytical quality control (QC) external pooled urine samples were also transferred to 2.0mL pre-labeled LoBind Eppendorf tubes. All samples were mixed with 300uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH in D2O. The tubes were vortexed for 5 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | Described in summary |
