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MB Sample ID: SA150509
| Local Sample ID: | DRB18invivo_12 |
| Subject ID: | SU001715 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 4 weeks mice |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001721 |
| Sampleprep Summary: | Tumors were homogenized in a Beadbud homogenizer (Benchmark scientific) in a mixture of LC-MS grade ice-cold methanol and water (1:1; v/v). The supernatant was collected and sonicated in a water bath incubator for 15 minutes, followed by centrifugation at 13,000 rpm for 10 minutes and collection of the supernatant. Supernatants collected from in vitro and in vivo extraction were then lyophilized using a speed vacuum evaporator. The samples were then dissolved into a mixture of LC-MS grade acetonitrile/water (1:1; v/v) for analysis. |
| Sampleprep Protocol Filename: | prats1988_20201207_Invivo_collection_and_preparation_method.docx |
| Processing Method: | Metabolite extraction; Quenching; speed vacuum evaporation |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Quenching with ice cold methanol |
| Extract Enrichment: | Speed vaccum evaporator |
| Sample Resuspension: | Acetonitrile/waster (1:1) |
