Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA151726

Local Sample ID:	Inulin_Media_2
Subject ID:	SU001728
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Female

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Sample Preparation:

Sampleprep ID:	SP001734
Sampleprep Summary:	The collected medium was weighed, and equal amounts of medium (50% of total medium weight) were accurately aliquoted from each sample and lyophilized. The microbial cells were quenched using 450 μL cold methanol right after collection. After brief agitation by vortex, the samples were transferred into glass tubes. Then 5 mL of methyl tert-butyl ether was added to each tube and the samples were mixed on a multi-tube vortexer (600 rpm, 30 min). Phase separation was then induced by adding 1.25 mL of Millipore deionized water. Samples were then vortexed again for 1 min, incubated at 4 °C for 10 min to allow phase separation and centrifuged (3,000 g, 10 min, 4 °C). Polar fractions were collected into clean tubes and lyophilized.

Sampleprep ID:

SP001734

Sampleprep Summary:

The collected medium was weighed, and equal amounts of medium (50% of total medium weight) were accurately aliquoted from each sample and lyophilized. The microbial cells were quenched using 450 μL cold methanol right after collection. After brief agitation by vortex, the samples were transferred into glass tubes. Then 5 mL of methyl tert-butyl ether was added to each tube and the samples were mixed on a multi-tube vortexer (600 rpm, 30 min). Phase separation was then induced by adding 1.25 mL of Millipore deionized water. Samples were then vortexed again for 1 min, incubated at 4 °C for 10 min to allow phase separation and centrifuged (3,000 g, 10 min, 4 °C). Polar fractions were collected into clean tubes and lyophilized.