Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA160374

Local Sample ID:	1205
Subject ID:	SU001789
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Age Or Age Range:	6–8 weeks old
Gender:	Female
Animal Animal Supplier:	Charles River Frederick Research Model Facility

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Subject
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Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP001795
Sampleprep Summary:	Blood samples were separated into plasma and packed cells by centrifugation at 3,500g for 15 min at 4°C and stored at -80°C until extraction. 35 µL of plasma were extracted in a water:methanol:chloroform mixture. Centrifuged for 20 min at 4°ᵒC and 13,000 rpm and the resulting upper hydrophilic phase was then transferred to a clean vial and dried under a stream of N2 gas. Dried sediments were resuspended in 60% methanol (aq.) and injected into the LCMS system.

Sampleprep ID:

SP001795

Sampleprep Summary:

Blood samples were separated into plasma and packed cells by centrifugation at 3,500g for 15 min at 4°C and stored at -80°C until extraction. 35 µL of plasma were extracted in a water:methanol:chloroform mixture. Centrifuged for 20 min at 4°ᵒC and 13,000 rpm and the resulting upper hydrophilic phase was then transferred to a clean vial and dried under a stream of N2 gas. Dried sediments were resuspended in 60% methanol (aq.) and injected into the LCMS system.