Return to study ST001717 main page
MB Sample ID: SA161871
Local Sample ID: | Mito_003_ne |
Subject ID: | SU001794 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | HeLa cell line |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001800 |
Sampleprep Summary: | For mitochondrial analysis, isolated mitochondria from 2x10^7 cells were mixed with 500 µl of 100 mM hydrochloric acid solution in methanol/water (8:2, v/v), 700 µl of chloroform and 500 µl of 100 mM hydrochloric acid solution in water. The mitochondrial sample was homogenized with 2.8 mm zirconium oxide beads for 5 min. After centrifugation at 30,130 xg and 4°C for 15 min, 600 µl of the lower phase was collected. Both the cell and mitochondrial extracts were dried under a gentle nitrogen stream at room temperature and reconstituted in 300 µL of isopropanol/acetonitrile/water (2:1:1, v/v/v). Eighty microliters of each sample was mixed with 20 µL of SPLASH LIPIDOMIX internal standard mix (Avanti Polar Lipids, USA). Finally, 5 µl of each sample was injected into the UPLC-QTOF MS system. |