Return to study ST001721 main page
MB Sample ID: SA162060
| Local Sample ID: | MH2_3 |
| Subject ID: | SU001798 |
| Subject Type: | Invertebrate |
| Subject Species: | Cherax tenuimanus |
| Taxonomy ID: | 99755 |
| Weight Or Weight Range: | 116g-198g |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001804 |
| Sampleprep Summary: | The dried haemolymph samples were reconstituted using 100µL of LC-MS water containing 0.1% Formic acid. Samples were manually swirled, then placed in a thermomixer for 2 minutes at 4oC, before being centrifuged at (1800 g) for 5 minutes at 4oC. Next, 40µL of the supernatant was transferred into LC-MS amber vials with inserts and placed in the autosampler kept at 6oC. The order in which samples were analysed was randomised to avoid any potential instrument bias. A pooled quality control (QC) sample was prepared by adding 40 µL from each reconstituted sample to a single Eppendorf tube, which was then mixed to homogenise in a thermal mixer and centrifuged as above. This pooled sample was aliquoted (40µL) into LC-MS amber vials to create 16 QC samples and placed into the autosampler tray kept at 6°C ready for analysis. Samples were analysed within 24 hours from preparation. At the start of the analytical batch, a solvent blank, matrix blank, and ten conditioning QC samples were analysed (Broadhurst et al., 2018). QC samples were then injected after every fifth marron haemolymph sample with two QCs analysed at the end of the batch, following the standard protocols for metabolomics (Broadhurst et al., 2018). |
| Processing Storage Conditions: | Described in summary |
