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MB Sample ID: SA162070
| Local Sample ID: | QC-1_neg_1a |
| Subject ID: | SU001799 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Age Or Age Range: | 0-125 days |
| Weight Or Weight Range: | 1-80 kg |
| Gender: | Male |
| Animal Animal Supplier: | Swine Innovation Centre Sterksel, the Netherlands |
| Animal Housing: | Metabolism cages |
| Animal Light Cycle: | 12h light / 12h darknes |
| Animal Feed: | Experimental diets (see publication) |
| Animal Water: | Ad libitum |
| Animal Inclusion Criteria: | Birth weight |
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Sample Preparation:
| Sampleprep ID: | SP001805 |
| Sampleprep Summary: | Plasma samples (150 µL) were pipetted into a 96-well plate and 450 µL acetonitrile containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL, was added per well. The samples were mixed immediately and placed at 4°C for 10 min for protein precipitation. After centrifugation (3700 rpm for 25 min at 4°C), the supernatant was transferred to a filter plate fixed on top of a 96-well collection plate in a manifold with a pressure gauge. Vacuum was then applied to the filter plate, and the solvent containing plasma components was collected into the 96-well collection plate. The collection plate was placed in a vacuum centrifuge and the samples were evaporated to dryness (ca. 2.5 h, 805 × g and 30°C). A volume of 150 µL of water/acetonitrile/formic acid (95/5/0.1%) was added to the dried extracts in the collection plate. The dried collection plate was then sealed with a piece of aluminum laminate (#186002789, Waters), centrifuged (3700 rpm for 25 min at 4°C), and kept in the auto-sampler at 10°C for the UPLC-QTOF/MS analysis. The injection volume was 3 µl. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
