Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA162302

Local Sample ID:	40
Subject ID:	SU001801
Subject Type:	Mammal
Subject Species:	Sus scrofa
Taxonomy ID:	9823
Age Or Age Range:	0-125 days
Weight Or Weight Range:	1-80 kg
Gender:	Male
Animal Animal Supplier:	Swine Innovation Centre Sterksel, the Netherlands
Animal Housing:	Metabolism cages
Animal Light Cycle:	12h light / 12h darknes
Animal Feed:	Experimental diets (see publication)
Animal Water:	Ad libitum
Animal Inclusion Criteria:	Birth weight

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Sample Preparation:

Sampleprep ID:	SP001807
Sampleprep Summary:	In a 96-well plate, the urine samples (112.5 µL) were diluted with H2O (112.5 µL) and 25 µL acetonitrile (100%, ACN) containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL was added. The samples were incubated for 20 min at 4°C and subsequently centrifuged (3700 rpm, 25 min, 4°C). The supernatant, 100 µL per well, was transferred to a 200 µL 96-well plate and a protective film was welded on the plate using a heat sealer, and the plate was centrifuged at 3700 rpm, 4°C for 25 min prior to the LC-MS analysis. Samples were kept in the autosampler at 10°C, and the injection volume was 3 µl.

Sampleprep ID:

SP001807

Sampleprep Summary:

In a 96-well plate, the urine samples (112.5 µL) were diluted with H2O (112.5 µL) and 25 µL acetonitrile (100%, ACN) containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL was added. The samples were incubated for 20 min at 4°C and subsequently centrifuged (3700 rpm, 25 min, 4°C). The supernatant, 100 µL per well, was transferred to a 200 µL 96-well plate and a protective film was welded on the plate using a heat sealer, and the plate was centrifuged at 3700 rpm, 4°C for 25 min prior to the LC-MS analysis. Samples were kept in the autosampler at 10°C, and the injection volume was 3 µl.