Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA175493

Local Sample ID:	1056108115
Subject ID:	SU001967
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP001973
Sampleprep Summary:	Plasma samples were thawed on ice, prepared and analyzed randomly as previously described (Contrepois et al., 2015, Contrepois et al., 2018). Briefly, metabolites were extracted using 1:1:1 acetone:acetonitrile:methanol, evaporated to dryness under nitrogen and reconstituted in 1:1 methanol:water before analysis. Fifteen labeled metabolite internal standards (IS) were added to each sample to control for extraction efficiency and LC-MS performance. Complex lipids were prepared using a biphasic separation with cold methyl tert-butyl ether (MTBE), methanol and water and reconstituted in 9:1 methanol:toluene. Each sample was spiked-in with deuterated lipid IS (Sciex, cat#: 5040156) used for quantification. For quality controls, 3 reference plasma samples (40 µl plasma) and 1 preparation blank were processed in parallel.

Sampleprep ID:

SP001973

Sampleprep Summary:

Plasma samples were thawed on ice, prepared and analyzed randomly as previously described (Contrepois et al., 2015, Contrepois et al., 2018). Briefly, metabolites were extracted using 1:1:1 acetone:acetonitrile:methanol, evaporated to dryness under nitrogen and reconstituted in 1:1 methanol:water before analysis. Fifteen labeled metabolite internal standards (IS) were added to each sample to control for extraction efficiency and LC-MS performance. Complex lipids were prepared using a biphasic separation with cold methyl tert-butyl ether (MTBE), methanol and water and reconstituted in 9:1 methanol:toluene. Each sample was spiked-in with deuterated lipid IS (Sciex, cat#: 5040156) used for quantification. For quality controls, 3 reference plasma samples (40 µl plasma) and 1 preparation blank were processed in parallel.