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MB Sample ID: SA186773
| Local Sample ID: | SA184026 |
| Subject ID: | SU002076 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 679895 |
| Genotype Strain: | BW25113 |
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Sample Preparation:
| Sampleprep ID: | SP002082 |
| Sampleprep Summary: | LCMS samples from culture supernatant, were desalted and concentrated ~ 6 times using C18 stage tips before LCMS analysis. Stage tips were washed with 200 μL 100 % MeOH and equilibrated using 200 μL 95:5 H2O/MeCN + 0.1 % FA and centrifugation at 3000 rcf. Subsequently, 400 - 500 μL of cleared supernatant were applied to the stage tips and the C18 matrix was washed with 30 μL 95:5 H2O/MeCN + 0.1 % FA to remove salts. Finally, the samples were eluted using two times 30 μL 20:80 H2O/MeCN + 0.1 % FA. For LCMS analysis of the pellet, cells were harvested by centrifugation and the pellet was resuspended in 10 % of the harvested volume 20:80 H2O/MeCN + 0.1 % FA. The resulting suspension was sonicated for 10 min. Subsequently any cell debris was removed by centrifugation at maximum speed for 10 min. |
| Sampleprep Protocol Filename: | Sample Preparation Protocol_Mass_Spectrometry.docx |
