Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA207562

Local Sample ID:	DT2
Subject ID:	SU002250
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002256
Sampleprep Summary:	1x108 293FT, TMEM41B KO and VMP1 KO cells were washed with PBS thrice before adding 560 µl extraction solvent (methanol:water = 2:5), pre-cooled at −4°C. Cells were then scraped into the extraction solvent on ice and transferred into eppendorf tubes. Then, 800 µL methyl tert-butyl ether (MTBE) was added and the samples were sonicated for 10 min. After centrifugation for 15 min at 3,000 rpm at 4°C to separate phases, the upper layer and lower layer were collected for lipidomics and metabolomics analysis, respectively.

Sampleprep ID:

SP002256

Sampleprep Summary:

1x108 293FT, TMEM41B KO and VMP1 KO cells were washed with PBS thrice before adding 560 µl extraction solvent (methanol:water = 2:5), pre-cooled at −4°C. Cells were then scraped into the extraction solvent on ice and transferred into eppendorf tubes. Then, 800 µL methyl tert-butyl ether (MTBE) was added and the samples were sonicated for 10 min. After centrifugation for 15 min at 3,000 rpm at 4°C to separate phases, the upper layer and lower layer were collected for lipidomics and metabolomics analysis, respectively.