Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA211999

Local Sample ID:	MS58_018
Subject ID:	SU002307
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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MS

Sample Preparation:

Sampleprep ID:	SP002313
Sampleprep Summary:	The day of the extraction, cells were washed at room temperature with PBS twice and then kept on cold bath with dry ice and methanol. Metabolite extraction solution (50% methanol, 30% acetonitrile, 20% ultrapure water, 5 µM final concentration valine-d8) was added to each well following the proportion of 1 ml of extraction solution per million cells. The extracts were scraped and mixed at 4°C for 15 min. After final centrifugation at max speed for 15 min at 4°C, the supernatants were transferred into LC-MS vials.

Sampleprep ID:

SP002313

Sampleprep Summary:

The day of the extraction, cells were washed at room temperature with PBS twice and then kept on cold bath with dry ice and methanol. Metabolite extraction solution (50% methanol, 30% acetonitrile, 20% ultrapure water, 5 µM final concentration valine-d8) was added to each well following the proportion of 1 ml of extraction solution per million cells. The extracts were scraped and mixed at 4°C for 15 min. After final centrifugation at max speed for 15 min at 4°C, the supernatants were transferred into LC-MS vials.