Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA242832

Local Sample ID:	D15
Subject ID:	SU002517
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	18-65
Gender:	Male and female
Human Exclusion Criteria:	HIV patients, respiratory infections beside TB, diabetes, chronic renal failure history and transplanted. individuals

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Sample Preparation:

Sampleprep ID:	SP002523
Sampleprep Summary:	Serum samples (100 µL) were extracted with 300 µL of methanol overnight at 4°C. The morning after, the samples were centrifuged for 5 min at 16.000 ×g and 4°C. The supernatant was transferred to a new tube, evaporated in the speedvac, and the pellet was stored at −20°C until further analysis. Previous to LC-MS/MS analysis the pellets were resuspended in 99.16 µL of H2O 0.1%FA. The Internal Standard (IS) – 3-Nitro-L-tyrosine – was added to a final concentration of 42 µM. In addition, four pools of samples were used for metabolite identification.

Sampleprep ID:

SP002523

Sampleprep Summary:

Serum samples (100 µL) were extracted with 300 µL of methanol overnight at 4°C. The morning after, the samples were centrifuged for 5 min at 16.000 ×g and 4°C. The supernatant was transferred to a new tube, evaporated in the speedvac, and the pellet was stored at −20°C until further analysis. Previous to LC-MS/MS analysis the pellets were resuspended in 99.16 µL of H2O 0.1%FA. The Internal Standard (IS) – 3-Nitro-L-tyrosine – was added to a final concentration of 42 µM. In addition, four pools of samples were used for metabolite identification.