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MB Sample ID: SA256263
| Local Sample ID: | GALT_NR18_21756973 |
| Subject ID: | SU002652 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP002658 |
| Sampleprep Summary: | Metabolites extraction The polar metabolites were extracted from DBS samples using our developed standard protocol (Jacob et al., 2018). Five 3 mm size DBS disks were used for metabolite extraction using methanol, acetonitrile, and water (40:40:20%) for protein precipitation. The mixture was mixed at 25°C and 600 rpm for 2 hours in a thermomixer (Eppendorf, Germany). Pooled QC samples were prepared using aliquots from the study samples. Afterward, the supernatants were transferred to another set of tubes, evaporated in SpeedVacc (Christ, City, Germany), and stored at −80°C until LCMS analysis. |
| Sampleprep Protocol Filename: | Metabolites extraction |
| Processing Storage Conditions: | -20℃ |
| Extract Storage: | Room temperature |
