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MB Sample ID: SA006655

Local Sample ID:L14
Subject ID:SU000140
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:20 weeks
Weight Or Weight Range:26.8 +/- 2.2 g
Gender:Male
Animal Animal Supplier:Jackson Labs (Bar Harbor, ME)
Animal Housing:8 / cage
Animal Light Cycle:12 h / 12 h light/dark
Animal Feed:Standard chow, ad lib.
Animal Water:Ad lib. Access
Species Group:Mammal

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Treatment:

Treatment ID:TR000142
Treatment Summary:Mice were prepared or tissue collection by one of 6 modes of anesthesia or euthanasia, then tissues were rapidly surgically collected and frozen
Animal Anesthesia:One of 6 methods: 1) Cervical dislocation, 2) Carbon dioxide inhalation, 3) Isoflurane overdose, 4) continuous isoflurane, 5) ketamine, 6) pentobarbital
Animal Fasting:5 hours
Animal Endp Euthanasia:All tissue collection were terminal studies; in methods 1-3, animals were euthanized prior to tissue collection; in methods 4-6, animals were under deep anesthesia while tissues were collected.
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