Return to study ST000134 main page
MB Sample ID: SA007396
Local Sample ID: | SeM (PNT1A) |
Subject ID: | SU000153 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC, Sigma |
Cell Strain Details: | DU145 prostate cancer, PNT1A prostate nontumor |
Subject Comments: | 10 |
Cell Primary Immortalized: | Immortalized |
Cell Passage Number: | 10 |
Cell Counts: | 10 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR000155 |
Treatment Summary: | Cells were grown to 80% confluence in Petri dishes (50 mm in diameter) in 4.5% CO2 at 37 OC for 5-6 days for full attachment to the substratum. After aspirating the old growth medium, 3 mL of SeM or SeMSC (300 mM) in fresh pre-warmed medium at 37 ÂșC was added to each dish. |
Treatment Compound: | Selenomethionine / Se-methylselenocysteine |
Treatment Dose: | 300 mM |
Treatment Dosevolume: | 3 mL |
Treatment Doseduration: | 24 hrs |
Treatment Vehicle: | dissolved in culture media |
Cell Storage: | incubator |
Cell Growth Container: | petri dish (Corning) |
Cell Growth Config: | monolayer, adherent |
Cell Growth Rate: | 5-6 days |
Cell Inoc Proc: | trypsinized, then subculture |
Cell Media: | RPMI, EMEM |
Cell Envir Cond: | 37 C, 4.5% CO2 incubator |
Cell Harvesting: | after trypsinization |
Cell Pct Confluence: | 80% |
Cell Media Lastchanged: | 2-3 days |