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MB Sample ID: SA007397
| Local Sample ID: | SeMSC (DU145) |
| Subject ID: | SU000153 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC, Sigma |
| Cell Strain Details: | DU145 prostate cancer, PNT1A prostate nontumor |
| Subject Comments: | 10 |
| Cell Primary Immortalized: | Immortalized |
| Cell Passage Number: | 10 |
| Cell Counts: | 10 |
| Species Group: | Human |
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Treatment:
| Treatment ID: | TR000155 |
| Treatment Summary: | Cells were grown to 80% confluence in Petri dishes (50 mm in diameter) in 4.5% CO2 at 37 OC for 5-6 days for full attachment to the substratum. After aspirating the old growth medium, 3 mL of SeM or SeMSC (300 mM) in fresh pre-warmed medium at 37 ºC was added to each dish. |
| Treatment Compound: | Selenomethionine / Se-methylselenocysteine |
| Treatment Dose: | 300 mM |
| Treatment Dosevolume: | 3 mL |
| Treatment Doseduration: | 24 hrs |
| Treatment Vehicle: | dissolved in culture media |
| Cell Storage: | incubator |
| Cell Growth Container: | petri dish (Corning) |
| Cell Growth Config: | monolayer, adherent |
| Cell Growth Rate: | 5-6 days |
| Cell Inoc Proc: | trypsinized, then subculture |
| Cell Media: | RPMI, EMEM |
| Cell Envir Cond: | 37 C, 4.5% CO2 incubator |
| Cell Harvesting: | after trypsinization |
| Cell Pct Confluence: | 80% |
| Cell Media Lastchanged: | 2-3 days |
