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MB Sample ID: SA013976
| Local Sample ID: | CD3 WT2 |
| Subject ID: | SU000327 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Animal Animal Supplier: | WT (CRBN +/+) mice are from Jackson labs and CRBN -/- mice have been bred at Moffitt Cancer Center |
| Animal Housing: | Standard bedding + cotton pads, hepa filtered air |
| Animal Light Cycle: | Dark 6pm-6am |
| Animal Feed: | Feed from Harlan, not sterile |
| Animal Water: | autoclaved H2O |
| Animal Inclusion Criteria: | Animals used in this experiment were sex-matched and age matched. The ages were 8-12 weeks old in both sets of experiments |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000341 |
| Treatment Summary: | - |
| Animal Anesthesia: | CO2 asphyxiation |
| Animal Acclimation Duration: | Mice from Jackson are subjected to a week-long acclimation period in our facility prior to experimental use. |
| Animal Endp Tissue Coll List: | Spleen into cell culture media |
| Cell Growth Container: | 96-well round-bottom plate that was coated with anti-CD3 antibody |
| Cell Growth Rate: | Murine T cells generally divide after 48-72 hours of stimulation. |
| Cell Media: | RPMI 1640 with 10% FBS, pen/strep, gentamycin, NEAA, Sodium pyruvate, fungizone (Amphotericin B), 2-mercaptoethanol |
| Cell Envir Cond: | 37°C, normoxic conditions |
