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MB Sample ID: SA015473

Local Sample ID:AB33_U_9
Subject ID:SU000373
Subject Type:Plant
Subject Species:Cynodon transvaalensis
Taxonomy ID:75728
Genotype Strain:AB03, AB33, and AB39
Age Or Age Range:4 month old plant clones
Subject Comments:(African Bermudagrass,C. transvaalensis)
Species Group:Plant

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Treatment:

Treatment ID:TR000387
Treatment Summary:Plants clones propagated by stolons were allowed to establish for 30 days in 4 cm diameter conetainers filled with sand. Fifty sting nematodes (mixed age and gender) were added to each conetainer and allowed to grow and reproduce for 90 days. Nematodes were then rinsed off of roots for quantification, and plant roots were frozen in liquid nitrogen and stored at -80C until they were freeze-dried.Freeze-dried roots were ground to a powder and then submitted for global metabolomic analysis.
Treatment:Biotic
Treatment Compound:Sting nematode, Belonolaimus longicaudatus
Plant Growth Support:sand in conetainers- 4 cm wide, 30 cm deep
Plant Growth Location:temperature controlled greenhouse
Plant Plot Design:complete randomized design
Plant Light Period:Natural lighting, ca. 11:13 Light:Dark
Plant Humidity:ca.50%RH
Plant Temp:25°C
Plant Watering Regime:Twice daily for 5 min
Plant Nutritional Regime:Weekly liquid fertilizer of 24-8-16 (N-P-K)
Plant Estab Date:2015-09-24
Plant Harvest Date:2016-04-02
Plant Growth Stage:non-reproductive
Plant Metab Quench Method:liquid nitrogen, followed by storage at -80°C
Plant Harvest Method:rinsed roots were cut off the plant and placed into paper envelopes
Plant Storage:1)-80C until freeze-drying. 2) Held at room temperature in ziploc bags with silica packets. 3) Frozen in liquid nitrogen and ground using a Geno/Grinder 2010. 4) Transferred to 2 mL tubes and held at room temperature.
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